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ENCODE-cellranger

processing pipeline for ENCODE multiome datasets and GEO datasets (no fragment file available, FASTQ only)

datasets used for REMO performance analysis:

  • bile duct
  • fallopian tube
  • heart
  • left colon
  • liver
  • lung
  • muscle
  • pancreas
  • placenta

datasets used for REMO annotation:

  • pancreas1
  • pancreas2
  • pancreas3
  • pancreas4
  • retina1
  • retina2
  • retina3
  • retina4
  • retina5
  • retina6
  • retina7
  • retina8

dataset ID and FASTQ download SRA/link can be found in datasets.csv

software versions:

  • Cellranger-ARC 2.0.2
  • Reference version: 2020-A, Human GRCh38 (GENCODE v32/Ensembl98)

the provided ENCODE gex matrix contained decimals when it should just be integers, this pipeline downloads the FASTQ files and re-processes them using cellranger-arc

part of REMO project

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