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I am dealing with guide-seq for Cpf1. Is there any adjustment to be done on the pipeline for the “off-target calling” and “display” steps. Does the pipeline recognize the gRNA pattern with the PAM at the 5’?
Thanks for your help
I am dealing with guide-seq for Cpf1. Is there any adjustment to be done on the pipeline for the “off-target calling” and “display” steps. Does the pipeline recognize the gRNA pattern with the PAM at the 5’?
Thanks for your help