when I use seq2HLA, some errors occurred as follow:
First iteration starts....
Mapping ......
reads processed: 102029138
reads with at least one reported alignment: 0 (0.00%)
reads that failed to align: 102029138 (100.00%)
No alignments
Calculation of first digital haplotype.....
The digital haplotype is written into test-ClassI-nonclass.digitalhaplotype1
1st iteration done.
Now removing reads that mapped to the three top-scoring groups .......
Nothing to remove
Second iterations starts .....
Mapping ......
Error: reads file does not look like a FASTQ file
I have no idea why my fastq file of RNA-seq cannot be used. Any recommendations to solve my problems?
when I use seq2HLA, some errors occurred as follow:
First iteration starts....
Mapping ......
reads processed: 102029138
reads with at least one reported alignment: 0 (0.00%)
reads that failed to align: 102029138 (100.00%)
No alignments
Calculation of first digital haplotype.....
The digital haplotype is written into test-ClassI-nonclass.digitalhaplotype1
1st iteration done.
Now removing reads that mapped to the three top-scoring groups .......
Nothing to remove
Second iterations starts .....
Mapping ......
Error: reads file does not look like a FASTQ file
I have no idea why my fastq file of RNA-seq cannot be used. Any recommendations to solve my problems?